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Extraction of Methadone using strata X-C on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS

Sample Preparation Details

Part No:8B-S029-TBJ
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:1 ml of urine was diluted with 1 ml of 100 mM phosphate buffer (pH6.0)
Dry:2-3 mins @ 10" of Hg
Final Prep and Analysis: Inject: 5 µL on HPLC / Mass Spectrometer (MS) @ amu (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Methadone 1.5 
2 Methadone-d9 1.5 

Application Detail (App ID: 18649)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:0.1% Formic acid in H2O
Elution B:0.1% Formic acid in ACN
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 97 3
2 0.25 97 3
3 2.75 5 95
4 3 5 95
5 3.01 97 3
6 4.5 97 3

Flow Rate:500 µL/min
Col. Temp: 25 °C
Detection: Mass Spectrometer (MS) @ amu (22°C)
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
Methadone
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2
Methadone-d9
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