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Paracetamol Related substances using Kinetex 2.6µm C18 150 x 4.6mm ID

Application Detail (App ID: 18703)

Part No:00F-4462-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Isocratic
Elution A:100 mM Na2HPO4
Elution B:50 mM NaH2PO4
Elution C:4 g/L (Bu)4NOH in methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B Pct C
1 0 37.5 37.5 25

Flow Rate:0.9 mL/min
Col. Temp: 35 °C
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 245 nm (22°C)
Note:Mobile phase preparation:
A. dissolve 17.9g of disodium hydrogen phosphate in 1L of water
B. dissolve 7.8g of sodiumn dihydrogen phosphate in 1L of water
C. dissolve 400g of tetrabutyammonium hydroxide in 1L of water
D. mix 1 mL of (3) with 250 mL of methanol (4 g/L)
E. mix 375 mL of A + 375 mL of B+ 250 mL of D

Analyte Details

Analyte Detail not available

1
Impurity K (4-aminophenol)

2
Paracetamol
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3
impurity A (N-(2-hydroxyphenyl)acetamide)

4
Impurity I (1-(2-hydroxyphenyl)ethanone)

5
Impurity F (4-nitrophenol)

6
Impurity J (N-(4-chlorophenyl)acetamide (chloroacetanilide))