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Extraction of Healthcare Opiates using strata X-C on Kinetex 2.6µm C18 50 x 2.1mm ID by LC/MS

Sample Preparation Details

Part No:8B-S029-TBJ
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:1mL of Urine (spiked with analyte and IS, conc.= 300ng/mL) was diluted with 1 mL of 100mM
phosphate buffer (pH 6)
Dry:2 mins @ 10" of Hg pressure
Final Prep and Analysis: Blown down samples (after evaporation) were reconstituted in 200 uL of initial mobile phase (A/B=95/5). Inject: 5 µL on HPLC / Mass Spectrometer (MS) @ amu (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Normorphine 1.5 
2 Morphine 1.5 
3 Morphine-d3 1.5 
4 Oxymorphone 1.5 
5 Hydromorphone 1.5 
6 Hydromorphone-d6 1.5 
7 Codeine-d6 1.5 
8 Codeine 1.5 
9 O-Desmethyltramadol 1.5 
10 Oxycodone-d6 1.5 
11 Oxycodone 1.5 
12 Hydrocodone 1.5 
13 N-Desmethyltramadol 1.5 
14 Tramadol 1.5 
15 Normeperidine 1.5 
16 Normeperidine-d4 1.5 
17 Meperidine-d4 1.5 
18 Meperidine 1.5 

Application Detail (App ID: 18716)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:0.1% Formic acid and 5 mM ammonium acetate in H2O
Elution B:0.1% Formic acid and 5 mM ammonium acetate in 50-50ACN-Methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 95 5
2 0.24 95 5
3 3.36 20 80
4 3.37 5 95
5 3.68 5 95
6 3.69 95 5
7 5.55 95 5

Flow Rate:400 µL/min
Col. Temp: 25 °C
Detection: Mass Spectrometer (MS) @ amu (22°C)
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
Normorphine
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2
Morphine
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3
Morphine-d3
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4
Oxymorphone
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5
Hydromorphone
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6
Hydromorphone-d6
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7
Codeine-d6
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8
Codeine
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9
O-Desmethyltramadol
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10
Oxycodone-d6
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11
Oxycodone
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12
Hydrocodone
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13
N-Desmethyltramadol
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14
Tramadol
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15
Normeperidine
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16
Normeperidine-d4
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17
Meperidine-d4
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18
Meperidine
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