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Applications

Extraction of Healthcare Opiates using strata X-C on Kinetex 2.6µm C18 50 x 2.1mm ID by LC/MS

Sample Preparation Details

Condition 1 :1 mL Methanol at 2 mL/min
Condition 2 :1 mL Water at 2 mL/min
Load :Load 1.00 mL Sample diluted in 100 mM KH2PO4 (pH 6)
Wash 1 :1 mL 0.1N HCl at 2 mL/min
Wash 2 :1 mL Methanol at 2 mL/min
Dry :2 mins @ 10" of Hg pressure
Elution 1 :0.5 mL 5% ammonium hydroxide in methanol at 1 mL/min
Elution 2 :0.5 mL 5% ammonium hydroxide in methanol at 1 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :200 µL Initial mobile phase (A/B=95/5)
Notes :Blown down samples (after evaporation) were reconstituted in 200 uL of initial mobile phase (A/B=95/5).

Application Detail (App ID: 18716)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:0.1% Formic acid and 5 mM ammonium acetate in H2O
Elution B:0.1% Formic acid and 5 mM ammonium acetate in 50-50ACN-Methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 95 5
2 0.24 95 5
3 3.36 20 80
4 3.37 5 95
5 3.68 5 95
6 3.69 95 5
7 5.55 95 5

Flow Rate:400 µL/min
Col. Temp: 25 °C
Detection: Mass Spectrometer (MS) @ amu (22°C)
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
Normorphine
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2
Morphine
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3
Morphine-d3
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4
Oxymorphone
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5
Hydromorphone
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6
Hydromorphone-d6
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7
Codeine-d6
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8
Codeine
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9
O-Desmethyltramadol
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10
Oxycodone-d6
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11
Oxycodone
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12
Hydrocodone
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13
N-Desmethyltramadol
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14
Tramadol
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15
Normeperidine
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16
Normeperidine-d4
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17
Meperidine-d4
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18
Meperidine
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