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Extraction of Cocaine-NIDA5 Using strata X-C on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS

Sample Preparation Details

Part No:8B-S029-TBJ
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:1mL of Urine (spiked with analyte and IS, conc.= 150ng/mL) was diluted with 1 mL of 100mM
phosphate buffer (pH 6)
Dry:2 mins @ 10" of Hg pressure
Final Prep and Analysis: Blown down samples (after evaporation) were reconstituted in 200 uL of initial mobile phase (A/B=90/10). Inject: 5 µL on HPLC / Mass Spectrometer (MS) @ amu (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Benzoylecgonine-d3 0.75 
2 Benzoylecgonine 0.75 
3 Cocaine 0.75 
4 Cocaethylene 0.75 

Application Detail (App ID: 18843)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:0.1% Formic acid in H2O
Elution B:0.1% Formic acid in ACN
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 2 30 70
3 2.01 5 95
4 2.3 5 95
5 2.31 90 10
6 4.1 90 10

Flow Rate:450 µL/min
Col. Temp: 25 °C
Detection: Mass Spectrometer (MS) @ amu (22°C)
Detector Info: ESI Condition
Positive Ionization
NEB: 12.00
CUR: 7.00
CAD: 12.00
IS: 5500.00
TEM: 400.00
EP 10.00
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
Benzoylecgonine-d3
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2
Benzoylecgonine
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3
Cocaine
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4
Cocaethylene
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