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Extraction of Amphetamines-NIDA5 Using Strata X-C on Kinetex 2.6µm C18 50 x 2.1mm by LCMS

Sample Preparation Details

Condition 1 :1 mL Methanol at 2 mL/min
Condition 2 :1 mL Water at 2 mL/min
Load :Load 1.00 mL Sample diluted in 100 mM KH2PO4 (pH 6)
Wash 1 :1 mL 0.1N HCl at 2 mL/min
Wash 2 :1 mL Methanol at 2 mL/min
Dry :2 mins @ 10" of Hg pressure
Elution 1 :0.5 mL 5% ammonium hydroxide in methanol at 1 mL/min
Elution 2 :0.5 mL 5% ammonium hydroxide in methanol at 1 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :200 µL Initial mobile phase (A/B=90/10)
Notes :Blown down samples (after evaporation) were reconstituted in 200 uL of initial mobile phase (A/B=90/10).

Application Detail (App ID: 18845)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:0.1%FA-5mM NH4Ac in H2O
Elution B:0.1%FA-5mM NH4Ac in Methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 1 80 20
3 3 70 30
4 3.01 5 95
5 3.3 5 95
6 3.31 90 10
7 5.1 90 10

Flow Rate:450 µL/min
Col. Temp: 25 °C
Detection: Mass Spectrometer (MS) @ amu (22°C)
Detector Info: ESI Condition
Positive Ionization
NEB: 8.00
CUR: 8.00
IS: 5500.00
TEM: 0.00
CAD: 7.00
EP 10.00
Note:Start of Run - 3938.50 psi (271.55 bar)
End of Run - 5229.62 psi (360.57 bar)

Analyte Details

Analyte Detail not available

1
Norephedrine
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2
Ephedrine
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3
Pseudoephedrine
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4
Amphetamine-d11
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5
Amphetamine
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6
Methamphetamine-d14
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7
Methamphetamine
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8
Phentermine
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