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Extraction of Opiates-NIDA5 Using Strata X-C on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS

Sample Preparation Details

Part No:8B-S029-TBJ
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:1mL of Urine (spiked with analyte and IS, conc.= 2000ng/mL) was diluted with 1 mL of 100mM
phosphate buffer (pH 6)
Dry:2 mins @ 10" of Hg pressure
Final Prep and Analysis: Blown down samples (after evaporation) were reconstituted in 1mLof initial mobile phase (A/B=95/5). Inject: 15 µL on HPLC / Mass Spectrometer (MS) @ amu (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Morphine-d3
2 Morphine
3 Oxymorphone
4 Hydromorphone
5 Norcodeine
6 Codeine-d6
7 Oxycodone
8 Hydrocodone
9 Codeine

Application Detail (App ID: 18846)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:0.1% Formic acid in H2O
Elution B:0.1% Formic acid in ACN
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 95 5
2 0.24 95 5
3 1.25 70 30
4 2.5 70 30
5 2.51 5 95
6 2.8 5 95
7 2.81 95 5
8 4.6 95 5

Flow Rate:450 µL/min
Col. Temp: 25 °C
Detection: Mass Spectrometer (MS) @ amu (22°C)
Detector Info: ESI conditions
Positive Ionization
NEB: 12.00
CUR: 11.00
IS: 4500.00
TEM: 450.00
CAD: 11.00
EP 10.00
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
Morphine-d3
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2
Morphine
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3
Oxymorphone
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4
Hydromorphone
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5
Norcodeine
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6
Codeine-d6
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7
Oxycodone
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8
Hydrocodone
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9
Codeine
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