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Extraction of Expanded Amphetamines Using Strata X-C on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS

Sample Preparation Details

Part No:8B-S029-TBJ
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:1mL of Urine (spiked with analyte and IS, conc.= 500ng/mL) was diluted with 1 mL of 100mM
phosphate buffer (pH 6)
Dry:2 minutes at 10" of Hg pressure
Final Prep and Analysis: Blown down samples (after evaporation) were reconstituted in 1 mL of initial mobile phase (A/B=90/10). Inject: 5 µL on HPLC / Mass Spectrometer (MS) @ amu (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 HMMA 0.5 
2 Amphetamine-d11 0.5 
3 Amphetamine 0.5 
4 Methamphetamine 0.5 
5 Methamphetamine-d14 0.5 
6 MDA-d5 0.5 
7 MDA 0.5 
8 MDMA-d5 0.5 
9 MDMA 0.5 
10 MDEA-d5 0.5 
11 MDEA 0.5 

Application Detail (App ID: 18847)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:0.1%FA-5mM ammonium acetate in H2O
Elution B:0.1%FA-5mM ammonium acetate in Methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 3 60 40
3 3.02 5 95
4 3.3 5 95
5 3.31 90 10
6 5.1 90 10

Flow Rate:450 µL/min
Col. Temp: 25 °C
Detection: Mass Spectrometer (MS) @ amu (22°C)
Detector Info: ESI conditions
Positive Ionization
NEB: 12.00
CUR: 11.00
IS: 4500.00
TEM: 450.00
CAD: 11.00
EP 10.00
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
HMMA
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2
Amphetamine-d11
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3
Amphetamine
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4
Methamphetamine
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5
Methamphetamine-d14
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6
MDA-d5
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7
MDA
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8
MDMA-d5
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9
MDMA
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10
MDEA-d5
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11
MDEA
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