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Applications

Extraction of Steroids Using Strata-X on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS

Sample Preparation Details

Condition 1 :1 mL Methanol at 2 mL/min
Condition 2 :1 mL 0.1M Acetic acid at 2 mL/min
Load :Load 0.50 mL Sample diluted in 0.1M Acetic acid
Wash 1 :1 mL 0.1M Acetic acid at 2 mL/min
Wash 2 :1 mL 40% Methanol/water at 2 mL/min
Dry :2 mins at 10" of Hg pressure
Elution 1 :0.5 mL Methanol at 1 mL/min
Elution 2 :0.5 mL Methanol at 1 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :100 µL Initial mobile phase (A/B=80/20)
Notes :Blown down samples (after evaporation) were reconstituted in 100 uL of initial mobile phase (A/B=80/20).

Application Detail (App ID: 18848)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:0.1%FA in H2O
Elution B:0.1%FA in Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 80 20
2 2.5 20 80
3 2.51 5 95
4 2.8 5 95
5 2.81 80 20
6 4.6 80 20

Flow Rate:450 µL/min
Col. Temp: 25 °C
Detection: Mass Spectrometer (MS) @ amu (22°C)
Detector Info: ESI conditions
Positive Ionization
NEB: 10.00
CUR: 8.00
IS: 4500.00
TEM: 425.00
CAD: 7.00
EP 10.00
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
Triamcinolone
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2
Prednisolone
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3
Cortisone
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4
Betamethasone
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5
Corticosterone
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6
Triamcinolone acetonide
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7
11-alpha-Hydroxyprogesterone
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8
Cortisone acetate
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9
Testosterone
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10
11-Ketoprogesterone
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11
Betamethasone 17-valerate
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