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Extraction of Benzodiazepines Using Strata Screen-C on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS

Sample Preparation Details

Condition 1 :3 mL Methanol at 1 mL/min
Condition 2 :3 mL Water at 1 mL/min
Condition 3 :1 mL 100 mM phosphate buffer, pH 6 (adjust with phosphoric acid) at 1 mL/min
Load :Load 2.00 mL Sample diluted in 100 mm acetate buffer
Wash 1 :2 mL Water at 1 mL/min
Wash 2 :2 mL 20% acetonitrile/100 mm phosphate buffer, pH 6 at 1 mL/min
Dry :2-3 mins at 10" of Hg
Elution 1 :2 mL Ethylacetate at 1 mL/min
Elution 2 :2 mL Ethylacetate at 1 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :1 mL Initial mob phase(A/B=65/35)

Application Detail (App ID: 18849)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:0.1%FA in H2O
Elution B:0.1%FA in Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 65 35
2 2 65 35
3 2.01 5 95
4 2.3 5 95
5 2.31 65 35
6 4.1 65 35

Flow Rate:450 µL/min
Col. Temp: 25 °C
Detection: Mass Spectrometer (MS) @ amu (22°C)
Detector Info: ESI conditions
Positive Ionization
NEB: 12.00
CUR: 11.00
IS: 4500.00
TEM: 450.00
CAD: 11.00
EP 10.00
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
alpha-Hydroxy alprazolam
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2
alpha-HydroxyAlprazolam-d5
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3
Oxazepam-d5
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4
Oxazepam
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5
Nordiazepam-d5
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6
Nordiazepam
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7
Lorazepam
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8
Clonazepam
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9
Temazepam-d5
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10
Temazepam
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11
Diazepam
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