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Extraction of Benzodiazepines Using Strata Screen-C on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS

Sample Preparation Details

Part No:8B-S016-EBJ
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 100 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:2 ml of Urine sample (spiked with analyte and IS at 100ng/mL) diluted with 100 mM phosphate buffer, pH 6.
Dry:2-3 mins at 10" of Hg
Final Prep and Analysis: Inject: 5 µL on HPLC / Mass Spectrometer (MS) @ amu (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 alpha-Hydroxy alprazolam 0.2 
2 alpha-HydroxyAlprazolam-d5 0.2 
3 Oxazepam-d5 0.2 
4 Oxazepam 0.2 
5 Nordiazepam-d5 0.2 
6 Nordiazepam 0.2 
7 Lorazepam 0.2 
8 Clonazepam 0.2 
9 Temazepam-d5 0.2 
10 Temazepam 0.2 
11 Diazepam 0.2 

Application Detail (App ID: 18849)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:0.1%FA in H2O
Elution B:0.1%FA in Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 65 35
2 2 65 35
3 2.01 5 95
4 2.3 5 95
5 2.31 65 35
6 4.1 65 35

Flow Rate:450 µL/min
Col. Temp: 25 °C
Detection: Mass Spectrometer (MS) @ amu (22°C)
Detector Info: ESI conditions
Positive Ionization
NEB: 12.00
CUR: 11.00
IS: 4500.00
TEM: 450.00
CAD: 11.00
EP 10.00
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
alpha-Hydroxy alprazolam
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2
alpha-HydroxyAlprazolam-d5
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3
Oxazepam-d5
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4
Oxazepam
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5
Nordiazepam-d5
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6
Nordiazepam
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7
Lorazepam
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8
Clonazepam
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9
Temazepam-d5
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10
Temazepam
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11
Diazepam
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