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Applications

Pain panel extracted from human urine at cutoff - Kinetex C18 2.6µm 50x2.1mm LCMSMS

Sample Preparation Details

Condition 1 : 1 mL methanol
Condition 2 : 1 mL 100 mM ammonium acetate buffer (pH 5.95)
Load :Load Sample
Wash 1 : 2 x 1 mL 100 mM ammonium acetate buffer (pH 5.95)
Wash 2 : 2 x 1 mL methanol
Dry :5 mins under full vacuum
Elution 1 : 2 x 0.5 mL 60:24:16 ethyl acetate / IPA / NH4OH
Evaporate: :Evaporate to dryness
Reconstitute :1 mL 1 mL Initial Mobile Phase

Application Detail (App ID: 18850)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:0.1% Formic acid in H2O
Elution B:0.1% Formic acid in ACN
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 95 5
2 1.3 40 60
3 1.31 5 95
4 1.51 5 95
5 1.52 95 5
6 2.2 95 5

Flow Rate:1.2 mL/min
Col. Temp: 25 °C
Detection: Mass Spectrometer (MS) @ amu (22°C)
Note:ESI conditions
positive mode
QTrap 4000
NEB: 10.00
CUR: 11.00
IS: 4500.00
TEM: 450.00
CAD: 11.00
EP 10.00

Analyte Details

Analyte Detail not available

1
Morphine
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2
Morphine-d3
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3
Oxymorphone
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4
Hydromorphone
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5
Hydromorphone-d6
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6
Codeine
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7
Codeine-d6
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8
Naloxone
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9
Oxycodone
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10
Oxycodone-d6
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11
Naltrexone
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12
Hydrocodone
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13
cis-Tramadol
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14
C13-D3-tramadol

15
Meperidine
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16
Meperidine-d4
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17
Fentanyl
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18
Buprenorphine
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19
Buprenorphine-d4
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20
Methadone
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21
Methadone-d3
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