Clarity OTX- Recovery from Plasma

Sample Preparation Details

Part No:8E-S103-EGA
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 100 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Dry:N2 at 10u vaccuum
Final Prep and Analysis: Inject: 10 µL on HPLC / UV-Vis Abs.-Variable Wave.(UV) @ 260 nm (ambient)


Name Spiked
Log P pKa % Rec %RSC (n=0)
1 27 mer P-S Oligonucleotide 12 

Application Detail (App ID: 18868)

Part No:00F-4441-B0
Dimensions:150 x 2 mm ID
Elution Type:Gradient
Elution A:200mM HFIP/ 14mM TEA/ 2% MeOH/ water
Elution B:200mM HFIP/ 14mM TEA/ 98% MeOH/ water
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 5 85 15
3 20 35 65
4 20.1 5 95

Flow Rate:0.25 mL/min
Col. Temp: 40 °C
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 260 nm (22°C)
Detector Info: OD260 0.159, 12ug/ml 97% recovery
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Clarity Oligo-RP 4 x 2.0mm ID, 10/Pk Part No.: AJ0-8134
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

27 mer P-S Oligonucleotide