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Applications

Clarity OTX- Recovery Data- MS purity data of 27mer P-S peak from 18868

Sample Preparation Details

Condition 1 :0.5 mL MeOH at 1 mL/min
Condition 2 :0.5 mL Clarity OTX Equlibration Buffer at 1 mL/min
Sample Pretreatment :O
Load :Load 500.00 µL Sample diluted in 1:1 Clarity OTX loading buffer/ sample
Wash 1 :0.5 mL Clarity OTX wash solvent at 1 mL/min
Wash 2 :0.5 mL Clarity OTX equilibration Buffer at 1 mL/min
Dry :N2 at 10u vaccuum
Elution 1 :0.5 mL Clarity OTX elution buffer at 1 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :100 µL Aobile phase A

Application Detail (App ID: 18869)

Part No:00F-4441-B0
Dimensions:150 x 2 mm ID
Elution Type:Gradient
Elution A:200mM HFIP/ 14mM TEA/ 2% MeOH/ water
Elution B:200mM HFIP/ 14mM TEA/ 98% MeOH/ water
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 5 85 15
3 20 35 65
4 20.1 5 95

Flow Rate:0.25 mL/min
Col. Temp: 40 °C
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 260 nm (22°C)
Detector Info: OD260 0.159, 12ug/ml 97% recovery
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Clarity Oligo-RP 4 x 2.0mm ID, 10/Pk Part No.: AJ0-8134
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
27 mer P-S Oligonucleotide