Region

Applications

Mycotoxins on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS in ESI Positive Mode (1)

Sample Preparation Details

Condition 1 :3 mL Methanol at 1 mL/min
Condition 2 :3 mL Water at 1 mL/min
Condition 3 :1 mL 100 mM phosphate buffer, pH 6 (adjust with phosphoric acid) at 1 mL/min
Load :Load 2.00 mL Sample diluted in 100 mm acetate buffer
Wash 1 :2 mL Water at 1 mL/min
Wash 2 :2 mL 20% acetonitrile/100 mm phosphate buffer, pH 6 at 1 mL/min
Dry :2-3 mins at 10" of Hg
Elution 1 :2 mL Ethylacetate at 1 mL/min
Elution 2 :2 mL Ethylacetate at 1 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :1 mL Initial mob phase(A/B=65/35)

Application Detail (App ID: 18878)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:0.1% Formic acid
Elution B:0.1% Formic acid in Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 70 30
2 3 5 95
3 4 5 95
4 4.01 70 30
5 6 70 30

Flow Rate:400 µL/min
Col. Temp: 25 °C
Detection: Mass Spectrometer (MS) @ amu (22°C)
Detector Info: ESI conditions
Positive Ionization
NEB: 12.00
CUR: 11.00
IS: 4500.00
TEM: 450.00
CAD: 11.00
EP 10.00
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
Fumonisin B1
search

2
HT-2
search

3
T2-Toxin
search

4
Fumonisin B2
search

5
Ochratoxin
search