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Glibenclamide Related Substance HPLC Analysis (HP-EUR) on Kinetex 2.6u C18 50 x 4.6mm ID @ 1.2ml/min

Application Detail (App ID: 19021)

Part No:00B-4462-E0
Dimensions:50 x 4.6 mm ID
Elution Type:Gradient
Elution A:20:50:930 101.8g/L Triethylamine solution/ACN/H2O
Elution B:20:65:915 mobile phase A/H2O/ACN
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 45 55
2 15 45 55
3 30 5 95
4 40 5 95
5 41 45 55
6 55 45 55

Flow Rate:1.2 mL/min
Col. Temp: 35 °C
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 230 nm (22°C)
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 4.6mm ID Columns, 3/Pk Part No.: AJ0-8768
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
Glibenclamide impurity A

2
Glibenclamide impurity B

3
Glibenclamide
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4
Gliclazide
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