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Extraction of Analgesics from Plasma Using Strata X-A on Kinetex 2.6u, C18, 50x2.1mm by LC-UV

Sample Preparation Details

Condition 1 :1 mL Methanol at 1 mL/min
Condition 2 :1 mL Water at 1 mL/min
Load :Load 0.50 mL Sample diluted in Water
Wash 1 :1 mL 50 mm Na acetate, PH 7.0 at 1 mL/min
Wash 2 :1 mL Methanol at 1 mL/min
Dry :2 mins at 10" of Hg
Elution 1 :1 mL 5% FA/MeOH at 1 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :500 µL 20mm KH2PO4(PH 7.0)/ MeOH=90/10
Notes :Elution solvent was applied in 2 aliquots of 500uL followed by evaporation to dryness at 45 deg C under N2 and reconstitute with 500 uL initial mobile phase (20mm KH2PO4(PH 7.0)/ MeOH=90/10) spiked with 5ug/mL Fenoprofen (IS).

Application Detail (App ID: 19246)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:20 mM KH2PO4(pH 7.0)
Elution B:Methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 1 90 10
3 1.01 30 70
4 5.5 30 70

Flow Rate:0.4 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 230 nm (22°C)
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
Salicylic acid
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2
Naproxen
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3
Fenoprofen
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4
Ibuprofen
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