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Extraction of Analgesics from Plasma Using Strata X-A on Kinetex 2.6u, C18, 50x2.1mm by LC-UV

Sample Preparation Details

Part No:8B-S123-TAK
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:500 uL H Plasma (Na2 EDTA) spiked with Salicylic acid(5 ug/mL), Naproxen (2.5ug/mL) and Ibuprofen (5ug/mL) diluted with 500uL water, acidified with 2% H3PO4.
Dry:2 mins at 10" of Hg
Final Prep and Analysis: Elution solvent was applied in 2 aliquots of 500uL followed by evaporation to dryness at 45 deg C under N2 and reconstitute with 500 uL initial mobile phase (20mm KH2PO4(PH 7.0)/ MeOH=90/10) spiked with 5ug/mL Fenoprofen (IS).
Inject: 25 µL on HPLC / UV-Vis Abs.-Variable Wave.(UV) @ 230 nm (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Salicylic acid 90 2.3
2 Naproxen 2.5  90 0.66
3 Fenoprofen
4 Ibuprofen 99

Application Detail (App ID: 19246)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:20 mM KH2PO4(pH 7.0)
Elution B:Methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 1 90 10
3 1.01 30 70
4 5.5 30 70

Flow Rate:0.4 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 230 nm (22°C)
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
Salicylic acid
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2
Naproxen
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3
Fenoprofen
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4
Ibuprofen
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