Region

Applications

Extraction of Indole 3 carboxylic acid from Strata-X-A on Gemini 5u, C18, 150x4.6mm Column

Sample Preparation Details

Condition 1 :1 mL Methanol at 1 mL/min
Condition 2 :1 mL Water at 1 mL/min
Load :Load 500.00 µL Sample diluted in Water
Wash 1 :1 mL 50 mm Na acetate, PH 7.0 at 1 mL/min
Wash 2 :1 mL Methanol at 1 mL/min
Dry :2 mins at 10" of Hg
Elution 1 :1000 mL 5% FA/MeOH at 1 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :500 µL 20mm KH2PO4(PH 2.5)/ ACN=75/25
Notes :Elution solvent was applied in 2 aliquots of 500uL followed by evaporation to dryness at 45 deg C under N2 and reconstitute with 500 uL initial mobile phase (20mm KH2PO4(PH 2.5)/ ACN=75/25) spiked with 50ug/mL Salicylic acid (IS)

Application Detail (App ID: 19274)

Part No:00F-4435-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Isocratic
Elution A:20 mM KH2PO4(pH 2.5)
Elution B:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 75 25

Flow Rate:1 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 220 nm (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Gemini C18 4 x 3.0mm ID, 10/Pk Part No.: AJ0-7597
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Indole-3-carboxylic acid
search

2
Salicylic acid
search