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Chloramphenicol in Shrimp Using Strata-X and Kinetex C18, 50 x 2.1 mm by LCMS

Sample Preparation Details

Part No:8B-S100-UBJ
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 60 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Dry:5min @ >10 in Hg
Final Prep and Analysis: Inject: 25 µL on HPLC / Mass Spectrometer (MS) @ (450 °C)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Chloramphenicol 25 
2 Chloramphenicol-d5 25 

Application Detail (App ID: 19302)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:5 mM Ammonium bicarbonate
Elution B:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 95 5
2 2 95 5
3 2.01 5 95
4 4.5 5 95

Flow Rate:0.4 mL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ (450°C)
Detector Info: AB Sciex API 4000™
Note:Representative ion chromatogram (top) for chloramphenicol analysis (5 ng/mL extracted standard); 326.1/157.0 (IS, green), 321.2/152 (blue), and 321.2/257.2 (red) with zoomed in view of chromatogram shown at bottom.

Analyte Details

Analyte Detail not available

1
Chloramphenicol
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2
Chloramphenicol-d5
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