Region

Applications

0.1 ng/g Chloramphenicol in Shrimp using Strata-X and Kinetex C18 50 x 2.1 mm by LCMS

Sample Preparation Details

Part No:8B-S100-UBJ
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 60 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Dry:5min @ >10 in Hg
Final Prep and Analysis: Inject: 25 µL on HPLC / Mass Spectrometer (MS) @ (450 °C)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Chloramphenicol 0.1 
2 Chloramphenicol-d5 25 
3 impurity

Application Detail (App ID: 19453)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:5 mM Ammonium bicarbonate
Elution B:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 95 5
2 2 95 5
3 2.01 5 95
4 4.5 5 95

Flow Rate:0.4 mL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ (450°C)
Detector Info: AB Sciex API 4000™
Note:Extracted ion chromatogram for the lowest level (0.1 ng/mL or 0.01 ng/g of shrimp tissue) of extracted standard of chloramphenicol (321.2/152; top) and internal standard (326/157; bottom). The impurity peak eluting at about 3.0 minutes is well-resolved from chloramphenicol and does not affect quantitation.

Analyte Details

Analyte Detail not available

1
Chloramphenicol
search

2
Chloramphenicol-d5
search

3
impurity