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Tetrahydroiso-alpha acids extracted from beer by Strata-X using Kinetex C18 2.6µm 100x4.6 mm

Sample Preparation Details

Condition 1 :4 mL Acidified methanol (0.05% H3PO4) at 1 mL/min
Condition 2 :4 mL Water at 1 mL/min
Load :Load 5.00 mL Sample diluted in Miller Genuine Draft
Wash 1 :4 mL 40% methanol in water at 1 mL/min
Dry :5 min @ >10 inches of Hg
Elution 1 :2 mL Acidified methanol at 1 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :500 µL Mobile phase

Application Detail (App ID: 19486)

Part No:00D-4462-E0
Dimensions:100 x 4.6 mm ID
Elution Type:Isocratic
Elution A:Methanol
Elution B:Water
Elution C:Phosphoric acid
Gradient Profile:
Step No. Time (min) Pct A Pct B Pct C
1 0 75 24 1

Flow Rate:1.4 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 270 nm (22°C)
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 4.6mm ID Columns, 3/Pk Part No.: AJ0-8768
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
trans-Tetrahydroisocohumulone

2
cis-Tetrahydroisocohumulone

3
Tetrahydroisohumulone

4
Tetrahydroisoadhumulone