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Simvastatin by HPLC-Kinetex 2.6um XB-C18 100x4.6mm

Application Detail (App ID: 19520)

Part No:00D-4496-E0
Dimensions:100 x 4.6 mm ID
Elution Type:Isocratic
Elution A:65:35 Acetonitrile:28mM sodium phosphate pH 4.5
Gradient Profile:
Step No. Time (min) Pct A
1 15 100

Flow Rate:1.5 mL/min
Col. Temp: 45 °C
Detection: UV-Vis Abs.-Diode Array (PDA) @ 238 nm (22°C)
Note:For USP's system suitability requirements, the capacity factor for simvastatin must not be less than 3.0; column efficiency not less than 4500 theoretical plates; tailing factor not more than 2.0; resolution, while not specified according to USP standards, should be adequate at no less than 2.0. On the Kinetex 2.6um XB-C18 100x4.6mm, the capacity factor for simvastatin is approximately 3.0; column efficiency is 12,927; tailing factor is 1.01; and resolution between lovastatin and simvastatin is 8.36.

Analyte Details

Analyte Detail not available

1
Lovastatin
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2
Simvastatin
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