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Applications

Phencyclidine from urine using Strata-X-Drug B and Kinetex C18, 50 x 2.1 mm by LCMS

Sample Preparation Details

Condition 1 :2 at 2
Condition 2 :2 at 2
Load :Load 4.00 mL Sample diluted in Pre-treated Sample
Wash 1 : 2mL of 100mM Sodium acetate buffer (pH 5.0)
Wash 2 : 2mL of Methanol
Dry :10min @ >10 in Hg
Elution 1 : 2mL of Ethyl Acetate: IPA:NH4OH (7:2:1)
Evaporate: :Evaporate to dryness
Reconstitute :1 mL 20% methanol in water
Notes :Evaporate eluant to dryness and reconstitute in 1 mL of 20% methanol in water. Phencyclidine was spiked into the urine at the 25ng/mL cutoff.

Application Detail (App ID: 19595)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:0.1% Formic acid
Elution B:Methanol with 0.1% Formic Acid
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 75 25
2 1 5 95
3 2 5 95
4 2.1 75 25
5 4 75 25

Flow Rate:400 µL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ (500°C)
Note:Source temp: 500ºC
Polarity: Positive
Dwell: 100 msec

Analyte Details

Analyte Detail not available

1
Phencyclidine-d5
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2
Phencyclidine
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