Carboxy-THC from urine using Strata-X-Drug B and Zebron Drug-1 10x0.18x0.18

Sample Preparation Details

Part No:8B-S128-UCH
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 60 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:Pre-treatment: To each 2mL urine sample add 100µL of 100mM sodium acetate buffer with I.S. (300ng/mL) and 100µL of conc KOH. Incubate for 20min @ 60ºC. Cool and adjust pH to 4-5 using approx 450µL of glacial acetic acid. Load pre-treated sample directly - no conditioning required
Dry:15 min under full vacuum
Final Prep and Analysis: Evaporate eluant to dryness and reconstitute in 25µL ethyl acetate/50µL BSTFA with 1%TMCS. Incubate at 70ºC for 20min Inject: 1 µL on GC / Mass Selective (MSD) @ amu / (340 °C)


Name Spiked
Log P pKa % Rec %RSC (n=0)
1 Carboxy-THC-d9 15  100 0.01
2 Carboxy-THC 15  99.45 0.95

Application Detail (App ID: 19597)

Part No:7CD-G023-08
Phase:Proprietary Drug Phase
Dimensions:10 meters x 0.18 mm x 0.18 µm
Oven Profile:220ºC to 340ºC @ 30ºC/min
Carrier Gas:Constant Flow Helium, 0.7 mL/min
Injection:Split 10:1 1 µL @ 280°C
Detection: Mass Selective (MSD) (340°C)
Note:Analytes are spiked into blank urine @ the SAMSHA cutoff level of 15ng/mL.

After elution from SPE sorbent the sample is blown down and then derivatized with BSTFA by heating @ 70ºc for 20min.

Analyte Details

Analyte Detail not available