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Applications

Amphetamines by LC/MS/MS using Strata-X-Drug B and a Kinetex C18 2.6µm, 50x2.1mm

Sample Preparation Details

Part No:8B-S128-UCH
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 60 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:Pre-Treatment: To 2mL of urine add 1mL of 100mM phosphate buffer (pH 6.0) spiked with I.S. and 1mL of 0.35 M sodium periodate.
Mix/vortex and incubate at room temperature for 20 minutes. Load pre-treated sample directly - no conditioning required
Dry:10 min at 10 inches of Hg
Final Prep and Analysis: 1. Before blowing down eluent add 300µL of 0.5N methanolic hydrochloride (to prevent loss of analyte) 2. Dilute sample by a factor of 20 before injecting onto LCMS (Reconstitute in 10% methanol in formic acid (0.1%)). Inject: 2 µL on HPLC / Mass Spectrometer (MS) @ amu (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Amphetamine-d11 250  100 0.01
2 Amphetamine 250  101.8 1.59
3 Methamphetamine-d14 250  100 0.01
4 Methamphetamine 250  97.76 1.99
5 MDA-d5 250  100 0.01
6 MDA 250  96.65 3.03
7 MDMA-d5 250  100 0.01
8 MDMA 250  100.1 1.2
9 MDEA-d5 250  100 0.01
10 MDEA 250  102.2 1.93

Application Detail (App ID: 19598)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:0.1% Formic acid
Elution B:Methanol with 0.1% Formic acid
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 1 30 70
3 3 30 70
4 3 30 70

Flow Rate:400 µL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ amu (22°C)
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
Amphetamine-d11
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2
Amphetamine
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3
Methamphetamine-d14
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4
Methamphetamine
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5
MDA-d5
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6
MDA
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7
MDMA-d5
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8
MDMA
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9
MDEA-d5
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10
MDEA
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