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Applications

6-MAM by LC/MS/MS using Strata-X-Drug B and a Kinetex C18 2.6µm, 50x2.1mm

Sample Preparation Details

Load :Load 4.00 mL Sample diluted in 100mM Phosphate buffer
Wash 1 : 2mL of 100mM sodium acetate buffer pH 5.0
Wash 2 : 2mL of Methanol
Dry :10 min at 10 inches of Hg
Elution 1 : 2mL of Ethyl acetate: IPA:NH4OH (7:2:1)
Evaporate: :Evaporate to dryness
Reconstitute :1000 µL 10% methanol in formic acid (0.1%)
Notes :Evaporate eluant to dryness and reconstitute in 1mL of 10% methanol in formic acid (0.1%)

Application Detail (App ID: 19599)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:0.1% Formic acid
Elution B:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 1 30 70
3 3 30 70
4 3 30 70

Flow Rate:400 µL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ amu (22°C)
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
6-MAM-d3
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2
6-MAM
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