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Ibuprofen by HPLC on Luna 5um C18(2) 250x4.6mm

Application Detail (App ID: 19612)

Part No:00G-4252-E0
Dimensions:250 x 4.6 mm ID
Elution Type:Isocratic
Elution A:600:400 ACN:Water with 4g chloroacetic acid adjusted to pH 3.0 with ammonium hydroxide
Gradient Profile:
Step No. Time (min) Pct A
1 10 100

Flow Rate:2 mL/min
Col. Temp: 30 °C
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
Note:The USP Resolution requirement between any two peaks is not less than 2.5, and the tailing factor for any individual peak is not more than 2.5. On the LUNA 5um C18(2) the resolution between Ibuprofen and Valerophenone is 7.55; valerophenone and Ibuprofen related compound C is 6.11. The tailing factors for Ibuprofen, Valerophenone and Ibuprofen related compound C are 1.2, 1.45, and 1.26, respectively. The max back pressure was 215 bar.

Analyte Details

Analyte Detail not available

1
Ibuprofen
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2
Valerophenone
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3
Ibuprofen related compound C