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Applications
5 Steroid Mix spiked in Human Urine on Kinetex 2.6u XB-C18 75x4.60mm @ 2.00mL/min
19653
Separation Mode: Reversed Phase
Reversed Phase
XB-C18
HPLC
Anti-Inflammatory Agents
Clinical

Applications

5 Steroid Mix spiked in Human Urine on Kinetex 2.6u XB-C18 75x4.60mm @ 2.00mL/min
Analytes
1Hydrocortisone
2Cortisone
3Cortisone-21-acetate
417-Hydroxyprogesterone
5Deoxycorticosterone
Compound Name
Hydrocortisone
CID
5754
Molecular Formula
C21H30O5
Molecular Weight
362
No. Hydrogen Bond Acceptors
5
No. Hydrogen Bond Donors
3
Details
LC Conditions (App ID: 19653)
Column:
Brand Name:
Kinetex
Part No:
Phase Name:
XB-C18
Elution Type:
Gradient
Mobile Phase:
A: Water
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
107030
250100
Flow Rate:
2 mL/min
Temperature:
0°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) @ 220 nm (22°C)

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