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Fluoroquinolones by LCMS using Kinetex XB-C18 2.6µm, 50 x 2.1 mm

Sample Preparation Details

Condition 1 :3 mL Methanol at 2 mL/min
Condition 2 :3 mL Water at 2 mL/min
Load :Load 10.00 mL Sample diluted in Phosphate buffer with 2% TCA
Wash 1 :2 at 2
Dry :5min @ >10 in Hg
Elution 1 :2 mL Methanol/ACN/Acetic acid 49/49/2 at 1 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :500 µL 0.1% Formic acid

Application Detail (App ID: 19656)

Part No:00B-4496-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:0.1% Formic acid
Elution B:Methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 85 15
2 5 10 90
3 6 10 90
4 6 10 90

Flow Rate:0.4 mL/min
Col. Temp: 40 °C
Detection: Mass Spectrometer (MS) @ amu (550°C)
Detector Info: AB Sciex API 4000™
Note:Source temp: 550ºC
Polarity: Postive
Dwell: 25 msec

Analyte Details

Analyte Detail not available

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Ofloxacin
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