Applications

Fluoroquinolones by LCMS using Kinetex XB-C18 2.6µm, 50 x 2.1 mm

Analytes

1Ofloxacin
2Norfloxacin
3Norfloxacin-d5
4Ciprofloxacin
5Ciprofloxacin-d8
6Enrofloxacin
7Difloxacin
8Sparfloxacin

Compound Name Ofloxacin
CID 4583
Molecular Formula C18H20FN3O4
Molecular Weight 361
No. Chiral Centers: 1
No. Hydrogen Bond Acceptors 8
No. Hydrogen Bond Donors 1

Details

LC Conditions (App ID: 19656)

Column:

Kinetex 2.6 µm XB-C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand Name:

Kinetex

Part No:

00B-4496-AN

Phase Name:

XB-C18

Elution Type:

Gradient

Mobile Phase:

A: 0.1% Formic acid

B: Methanol

Gradient Profile:

Step No.	Time(min)	%A	%B
1	0	85	15
2	5	10	90
3	6	10	90
4	6	10	90

Flow Rate:

0.4 mL/min

Temperature:

40°C

Detection:

Mass Spectrometer (MS) (550°C)

Detector Info:

SCIEX API 4000™

Sample Note:

Source temp: 550ºC Polarity: Postive Dwell: 25 msec

Order Items Used in This Application

00B-4496-AN

Kinetex 2.6 µm XB-C18 100 Å, LC Column 50 x 2.1 mm, Ea

Qty:

infoSign in to view country specific pricing

Similar Applications

Select Analyte of Interest

Fluoroquinolones by LCMS using Kinetex C8 2.6µm, 50 x 2.1 mm

Kinetex 2.6 µm C8 100 Å, LC Column 50 x 2.1 mm, Ea

Reversed Phase

Fluoroquinolones from Propolis using Strata-X and Gemini-NX by LCMS

Gemini 3 µm NX-C18 110 Å, LC Column 50 x 2 mm, Ea

Reversed Phase

Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.

CONNECT WITH US

PRODUCTS AND SERVICES

SUPPORT

Product/Method Support

Product Troubleshooting

CQA / CofA

OUR COMPANY

About Phenomenex