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Ibuprofen by HPLC for Kinetex 2.6um XB-C18, 75x4.6mm

Application Detail (App ID: 19660)

Part No:00C-4496-E0
Dimensions:75 x 4.6 mm ID
Elution Type:Isocratic
Elution A:600:400 Acetonitrile: Water with 4g of chloroacetic acid adjusted to pH 3.0 with ammonium hydroxide
Gradient Profile:
Step No. Time (min) Pct A
1 10 100

Flow Rate:2 mL/min
Col. Temp: 30 °C
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
Note:The USP monograph specifies two system suitability requirements that must be met; these requirements are resolution and tailing factor: resolution between ibuprofen and valerophenone (the internal standard), and valerophenone and ibuprofen related compound C must not be less than 2.5; tailing factor for each peak must not be more than 2.5.
On the Kinetex 2.6um XB-C18 75x4.6mm column at a flow rate of 2.0mL/min
Rs between ibuprofen and valerophenone is 4.65
Rs between valerophenone and ibuprofen related compound C is 4.67; and Tf for ibuprofen, valerophenone, and ibuprofen related compound C are 1.71, 1.02 and 0.999, respectively

Analyte Details

Analyte Detail not available

1
Ibuprofen
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2
Valerophenone
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3
Ibuprofen related compound C