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Humic Acids using Kinetex C8 50x2.1 mm on ABI LC-MS 4000

Sample Preparation Details

Condition 1 :10 mL Dichloromethane at 5 mL/min
Condition 2 :20 mL Methanol at 5 mL/min
Condition 3 :20 mL MilliQ Water at 5 mL/min
Load :Load 100.00 mL Sample diluted in 75:25 MilliQ Water:Acetonitrile
Wash 1 :5 mL 50:50 Methanol:MilliQ Water at 2 mL/min
Dry :15 seconds
Elution 1 :6 mL Dichloromethane at 2 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :100 µL Dichlromethane

Application Detail (App ID: 19662)

Part No:00B-4497-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:5mM Ammoniium Acetate
Elution B:Methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 85 15
2 2 5 95
3 6 5 95
4 6.01 85 15

Flow Rate:0.4 mL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ 580.4 amu (22°C)
Detector Info: AB Sciex API 4000™
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C8 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8784
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
Humic acids
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