Humic Acids using Kinetex C8 50x2.1 mm on ABI LC-MS 4000

Sample Preparation Details

Part No:8B-S130-7CH
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 1.5 g bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Dry:15 seconds
Final Prep and Analysis: Inject: 5 µL on HPLC / Mass Spectrometer (MS) @ 580.4 amu (ambient)


Name Spiked
Log P pKa % Rec %RSC (n=0)
1 Humic acids

Application Detail (App ID: 19662)

Part No:00B-4497-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:5mM Ammoniium Acetate
Elution B:Methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 85 15
2 2 5 95
3 6 5 95
4 6.01 85 15

Flow Rate:0.4 mL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ 580.4 amu (22°C)
Detector Info: AB Sciex API 4000™
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C8 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8784
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

Humic acids