Region

Applications

Simvastatin by HPLC - Kinetex 2.6um C8 50x4.6mm

Application Detail (App ID: 19664)

Part No:00B-4497-E0
Dimensions:50 x 4.6 mm ID
Elution Type:Isocratic
Elution A:65:35 ACN: 28mM Monobasic Sodium phosphate pH 4.5
Gradient Profile:
Step No. Time (min) Pct A
1 15 100

Flow Rate:1.5 mL/min
Col. Temp: 45 °C
Detection: UV-Vis Abs.-Diode Array (PDA) @ 238 nm (22°C)
Note:For USP's system suitability requirements, the capacity factor for simvastatin must not be less than 3.0; column efficiency not less than 4500 theoretical plates; tailing factor not more than 2.0; resolution, while not specified according to USP standards, should be adequate at no less than 2.0. While the Kinetex 2.6um C8 50x4.6 does not meet the system suitability requirement for the capacity factor for simvastatin; the capacity factor is 1.4; it does have a column efficiency of 6,984; tailing factor of 1.12; and the resolution between lovastatin and simvastatin is 4.16.

Analyte Details

Analyte Detail not available

1
Lovastatin
search

2
Simvastatin
search