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Applications

Estrone, Estradiol, and Estriol from Water @ pH 2.0 Strata-X via Kinetex XB-C18 150x4.60mm

Sample Preparation Details

Condition 1 :5 mL Acetonitrile at 2 mL/min
Condition 2 :5 mL Methanol at 2 mL/min
Condition 3 :10 mL MilliQ Water at 2 mL/min
Load :Load 100.00 mL Sample diluted in Sample
Wash 1 : Not Required
Dry :1 Minute
Elution 1 :10 mL Acetonitrile at 2 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :1 mL Methanol

Application Detail (App ID: 19680)

Part No:00F-4496-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Gradient
Elution A:6.9mM Ammonium Acetate pH 4.0 with 25% Acetonitrile (v/v)
Elution B:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 100 0
2 10 35 65

Flow Rate:1.2 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 230 nm (22°C)
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 4.6mm ID Columns, 3/Pk Part No.: AJ0-8768
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
Estriol
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2
Ethyl paraben
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3
17-beta-Estradiol
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4
Estrone
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