Region

Applications

Estrone, Estradiol, and Estriol from Water @ pH 2.0 Strata-X via Kinetex XB-C18 150x4.60mm

Sample Preparation Details

Part No:8B-S100-FCH
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 200 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:100mLs of Water @ pH 2.0 spiked with Estrogen probes
Dry:1 Minute
Final Prep and Analysis: Inject: 5 µL on HPLC / UV-Vis Abs.-Variable Wave.(UV) @ 230 nm (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Estriol 30  81
2 Ethyl paraben 20  100
3 17-beta-Estradiol 30  98
4 Estrone 30  88

Application Detail (App ID: 19680)

Part No:00F-4496-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Gradient
Elution A:6.9mM Ammonium Acetate pH 4.0 with 25% Acetonitrile (v/v)
Elution B:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 100 0
2 10 35 65

Flow Rate:1.2 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 230 nm (22°C)
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 4.6mm ID Columns, 3/Pk Part No.: AJ0-8768
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
Estriol
search

2
Ethyl paraben
search

3
17-beta-Estradiol
search

4
Estrone
search