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Gabapentin extracted from plasma (0.5 µg/mL) using PPT on a Kinetex C18 2.6µm, 50x2.1mm

Application Detail (App ID: 19687)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:5 mM Ammonium formate
Elution B:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 97 3
2 1 97 3
3 3 10 90

Flow Rate:0.4 mL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ amu (22°C)
Detector Info: AB Sciex API 3000™
Note:Sample prep: Combine 100µL of plasma and 100µL of 10% TCA (containing internal standard at appropriate concentration) in a centrifuge vial. Mix briefly. Centrifuge samples at 14K RPM and then transfer 100µL of supernatant to an autosampler vial. Dilute sample 10X with water before injection.

Analyte Details

Analyte Detail not available

1
Gabapentin-d4
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2
Gabapentin
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