Region

Applications

LC/MS/MS Analysis of Digoxin and Digitoxin in plasma (0.5 ng/mL) using Strata-X and Kinetex C8

Sample Preparation Details

Condition 1 :2 mL Methanol at 2 mL/min
Condition 2 :2 mL 10mM Ammonium acetate at 2 mL/min
Load :Load 3.00 mL Sample diluted in Water
Wash 1 :1 mL 50:50 10mM ammonium acetate/10mM ammonium acetate in methanol at 2 mL/min
Dry :10min at full vacuum
Elution 1 :2 mL Methanol at 1 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :100 µL 50:50 Methanol/water

Application Detail (App ID: 19692)

Part No:00B-4497-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:10mM Ammonium acetate
Elution B:10mM Ammonium acetate in methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 50 50
2 2.5 0 100
3 2.51 50 50
4 5 50 50

Flow Rate:400 µL/min
Col. Temp: 30 °C
Detection: Mass Spectrometer (MS) @ amu (350°C)
Detector Info: AB Sciex API 4000™
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C8 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8784
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
Digoxin
search

2
Oleandrin
search

3
Digitoxin
search