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LC/MS/MS Analysis of Digoxin and Digitoxin in plasma (10 ng/mL) using Strata-X and Kinetex C8

Sample Preparation Details

Part No:8B-S100-TBJ
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Dry:10min at full vacuum
Final Prep and Analysis: Inject: 40 µL on HPLC / Mass Spectrometer (MS) @ amu (350 °C)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Digoxin 10  87
2 Oleandrin 10 
3 Digitoxin 10  108

Application Detail (App ID: 19694)

Part No:00B-4497-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:10mM Ammonium acetate
Elution B:10mM Ammonium acetate in methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 50 50
2 2.5 0 100
3 2.51 50 50
4 5 50 50

Flow Rate:400 µL/min
Col. Temp: 30 °C
Detection: Mass Spectrometer (MS) @ amu (350°C)
Detector Info: AB Sciex API 4000™
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C8 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8784
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
Digoxin
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2
Oleandrin
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3
Digitoxin
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