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Diclofenac from Plasma on Kinetex 2.6um C18 75x4.6mm

Sample Preparation Details

Part No:8B-S100-TAK
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:The plasma pre-treatment step comprised of filtration through a gauze cloth.
Afterwards, 500uL of diclofenac, which was dissolved in 5% methanol, was added to 500uL of plasma, and the solution mixture was then acidified with 600uL of 1M phosphoric acid.
Final Prep and Analysis: Inject: 20 µL on HPLC / UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Flurbiprofen 0.16 
2 Diclofenac 0.05 

Application Detail (App ID: 19701)

Part No:00C-4462-E0
Dimensions:75 x 4.6 mm ID
Elution Type:Isocratic
Elution A:63:37 Water(adjusted to pH 3.3 with phosphoric acid): Methanol
Gradient Profile:
Step No. Time (min) Pct A
1 5 100

Flow Rate:1.5 mL/min
Col. Temp: 30 °C
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 4.6mm ID Columns, 3/Pk Part No.: AJ0-8768
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
Flurbiprofen
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2
Diclofenac
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