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Diclofenac from Plasma on Kinetex 2.6um C18 75x4.6mm

Sample Preparation Details

Condition 1 :1 mL Methanol
Condition 2 :2 mL Water
Load :Load 1.60 mL Sample diluted in Methanol:Water
Wash 1 :1 mL 5% Methanol in Water
Elution 1 :1 mL Methanol
Evaporate: :Evaporate to dryness
Reconstitute :500 µL 37:63 Methanol:Water

Application Detail (App ID: 19701)

Part No:00C-4462-E0
Dimensions:75 x 4.6 mm ID
Elution Type:Isocratic
Elution A:63:37 Water(adjusted to pH 3.3 with phosphoric acid): Methanol
Gradient Profile:
Step No. Time (min) Pct A
1 5 100

Flow Rate:1.5 mL/min
Col. Temp: 30 °C
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 4.6mm ID Columns, 3/Pk Part No.: AJ0-8768
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
Flurbiprofen
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2
Diclofenac
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