Region

Applications

LC/MS/MS of Extended Opiates from urine using Strata-X-Drug B on a Kinetex C18 2.6µm, 50x2.1mm

Sample Preparation Details

Part No:8B-S128-UCH
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 60 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:To 2mL urine sample, add 500uL of conc. HCl. Heat at 90 C for 2 hours. Add 2mLs of 200mM Sodium Acetate Buffer (pH 4.0). Add 1mL of 6 N KOH. Vortex. Centrifuge for 5 mins at 5000rpm. Verify pH of sample is between 4.0-6.0. Load pre-treated sample directly onto SPE cartridge. NO CONDITIONING REQUIRED
Dry:10 minutes @ full vacuum
Final Prep and Analysis: Evaporate to dryness under a stream of nitrogen at 50 C . Reconstitute in 1 mL of 15% methanol. Inject: 5 µL on HPLC / Mass Spectrometer (MS) @ amu (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Oxymorphone-d3 300  100 0.001
2 Oxymorphone 300  99.4 0.52
3 Morphine-d3 300  100 0.001
4 Morphine 300  97.95 3.46
5 Hydromorphone-d6 300  100 0.001
6 O-Desmethyltramadol 300  101.89 1.61
7 Hydromorphone 300  98.31 1.84
8 Oxycodone-d6 300  100 0.001
9 Oxycodone 300  99.74 0.28
10 Codeine-d6 300  100 0.001
11 Codeine 300  102.46 2.12
12 N-Desmethyltramadol 300  100.3 1.4
13 cis-Tramadol 300  102.65 2.56
14 Hydrocodone-d6 300  100 0.001
15 Hydrocodone 300  99.39 0.88
16 Normeperidine-d4 300  100 0.001
17 Normeperidine 300  100.56 0.58
18 Meperidine-d4 300  100 0.001
19 Meperidine 300  100.21 0.35

Application Detail (App ID: 19705)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:5mM Ammonium acetate
Elution B:5mM Ammonium acetate in methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 85 15
2 5.5 0 100
3 6.5 0 100
4 6.51 85 15
5 9 85 15

Flow Rate:500 µL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ amu (22°C)
Detector Info: AB Sciex API 4000™

Source: Cur-25, CAD-7, IS-5500, Tem-600, GS1 60, GS2 55
Dwell: 20ms
Polarity: Positive
API 4000
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
Oxymorphone-d3
search

2
Oxymorphone
search

3
Morphine-d3
search

4
Morphine
search

5
Hydromorphone-d6
search

6
O-Desmethyltramadol
search

7
Hydromorphone
search

8
Oxycodone-d6
search

9
Oxycodone
search

10
Codeine-d6
search

11
Codeine
search

12
N-Desmethyltramadol
search

13
cis-Tramadol
search

14
Hydrocodone-d6
search

15
Hydrocodone
search

16
Normeperidine-d4
search

17
Normeperidine
search

18
Meperidine-d4
search

19
Meperidine
search