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Applications

LC/MS/MS of Extended Opiates from urine using Strata-X-Drug B on a Kinetex C18 2.6µm, 50x2.1mm

Sample Preparation Details

Load :Load Sample
Wash 1 : 2mLs of 100mM Sodium acetate (pH 5.0) buffer
Wash 2 : 2mLs of Methanol
Dry :10 minutes @ full vacuum
Elution 1 : 2mLs of Ethyl acetate:Isopropanol:NH4OH (7:2:1)
Evaporate: :Evaporate to dryness
Reconstitute :1 mL 15% methanol in water
Notes :Evaporate to dryness under a stream of nitrogen at 50 C . Reconstitute in 1 mL of 15% methanol.

Application Detail (App ID: 19705)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:5mM Ammonium acetate
Elution B:5mM Ammonium acetate in methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 85 15
2 5.5 0 100
3 6.5 0 100
4 6.51 85 15
5 9 85 15

Flow Rate:500 µL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ amu (22°C)
Detector Info: AB Sciex API 4000™

Source: Cur-25, CAD-7, IS-5500, Tem-600, GS1 60, GS2 55
Dwell: 20ms
Polarity: Positive
API 4000
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
Oxymorphone-d3
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2
Oxymorphone
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3
Morphine-d3
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4
Morphine
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5
Hydromorphone-d6
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6
O-Desmethyltramadol
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7
Hydromorphone
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8
Oxycodone-d6
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9
Oxycodone
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10
Codeine-d6
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11
Codeine
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12
N-Desmethyltramadol
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13
cis-Tramadol
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14
Hydrocodone-d6
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15
Hydrocodone
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16
Normeperidine-d4
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17
Normeperidine
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18
Meperidine-d4
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19
Meperidine
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