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Applications

Benzodiazepines from urine using Strata-X-Drug N on a Kinetex C18 2.6µm, 50x2.1mm

Sample Preparation Details

Load :Load Sample
Wash 1 : 2 mLs of Acetonitrile:Water (20:80) (v:v)
Dry :10 minutes @ full vacuum
Elution 1 : 2mLs of Ethyl acetate:Isopropanol (85:15) (v:v)
Evaporate: :Evaporate to dryness
Reconstitute :1 mL 35% methanol in water
Notes :Evaporate eluant under a stream of N2 gas at 50ºC. Reconstitute with 1mL of 35% methanol

Application Detail (App ID: 19714)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:0.1% Formic acid
Elution B:0.1% Formic acid in methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 65 35
2 4 5 95
3 4.01 65 35
4 7 65 35

Flow Rate:300 µL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ amu (22°C)
Detector Info: AB Sciex API 3000™

Source: Cur-11, CAD-12, IS-4500, Tem-450, NEB-12
Dwell: 25ms
Polarity: Positive
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
alpha-Hydroxyalprazolam
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2
Oxazepam-d5
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3
Oxazepam
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4
Alprazolam-d5
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5
Alprazolam
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6
Nordiazepam-d5
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7
Nordiazepam
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8
Lorazepam-d4
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9
Lorazepam
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10
Clonazepam-d4
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11
Clonazepam
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12
Temazepam-d5
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13
Temazepam
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14
Diazepam-d5
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15
Diazepam
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