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Applications

Barbiturates in urine using Strata-X-Drug N and Kinetex C18 2.6µm 100x2.1mm

Sample Preparation Details

Part No:8B-S129-ECH
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 100 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:Pre-Treatment: To 2mL of urine, add 2mLs of 100mM sodium acetate buffer (pH 5.0). pH should be ~4.0-6.0. Load directly onto sorbent - No Conditioning Required
Dry:10 min under full vacuum
Final Prep and Analysis: Evaporate eluant to dryness under stream of N2 (50ºC). Reconstitute samples with 1mL of 10% acetonitrile Inject: 10 µL on HPLC / Mass Spectrometer (MS) @ amu (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Phenobarbital-d5 300  100 0.01
2 Phenobarbital 300  99.64 0.48
3 Butalbital-d5 300  100 0.01
4 Butalbital 300  95.71 4.08
5 Pentobarbital 300  96.81 4.58
6 Amobarbital 300  96.38 4.34
7 Secobarbital 300  95.56 6.31

Application Detail (App ID: 19724)

Part No:00D-4462-AN
Dimensions:100 x 2.1 mm ID
Elution Type:Gradient
Elution A:5mM Ammonium acetate
Elution B:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
2 0 90 10
3 10 55 45
4 10.01 10 90
5 12 10 90
6 12.01 90 10
7 16 90 10

Flow Rate:400 µL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ amu (22°C)
Detector Info: AB Sciex API 5000™
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
Phenobarbital-d5
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2
Phenobarbital
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3
Butalbital-d5

4
Butalbital
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5
Pentobarbital
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6
Amobarbital
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7
Secobarbital
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