| 1Phenobarbital-d5 |
|---|
| 2Phenobarbital |
| 3Butalbital-d5 |
| 4Butalbital |
| 5Pentobarbital |
| 6Amobarbital |
| 7Secobarbital |
Analyte not found
Applications
Barbiturates in urine using Strata-X-Drug N and Kinetex C18 2.6µm 100x2.1mm
19724
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Adjuvants, Anesthesia
Clinical
Forensics/Toxicology
Analytes
Details
LC Conditions (App ID: 19724)
Column: | ||||||||||||||||||||||||||||
Brand Name: Kinetex | ||||||||||||||||||||||||||||
Part No: | ||||||||||||||||||||||||||||
Phase Name: C18 | ||||||||||||||||||||||||||||
Elution Type: Gradient | ||||||||||||||||||||||||||||
Mobile Phase: A: 5mM Ammonium acetate B: Acetonitrile | ||||||||||||||||||||||||||||
Gradient Profile:
| ||||||||||||||||||||||||||||
Flow Rate: 400 µL/min | ||||||||||||||||||||||||||||
Temperature: 0°C | ||||||||||||||||||||||||||||
Detection: Mass Spectrometer (MS) (22°C) | ||||||||||||||||||||||||||||
Detector Info: SCIEX API 5000™ |
Sample Preparation Details
Description: Strata-X-Drug N 100u Polymer RP, 100mg/6mL, 30/Pk |
Part Number: |
Pre Treatment Note: Evaporate eluant to dryness under stream of N2 (50ºC). Reconstitute samples with 1mL of 10% acetonitrile |
Sample PreTreatment: Evaporate eluant to dryness under stream of N2 (50ºC). Reconstitute samples with 1mL of 10% acetonitrile |
Wash 1: 0.1N HCl |
Wash 2: Methanol/0.1N HCl (30:70) |
Evaporation: Evaporate to dryness |
1 Shake Ethylacetate/IPA (85:15) |
1 Wash 0.1N HCl |
2 Wash Methanol/0.1N HCl (30:70) |
Order Items Used in This Application
00D-4462-AN
Kinetex 2.6 µm C18 100 Å, LC Column 100 x 2.1 mm, Ea
Qty:
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