Region

Applications

Barbiturates in urine using Strata-X-Drug N and Kinetex C18 2.6µm 100x2.1mm

Sample Preparation Details

Load :Load Sample
Wash 1 : 2 mLs of 0.1N HCl
Wash 2 : 2 mLs of Methanol/0.1N HCl (30:70)
Dry :10 min under full vacuum
Elution 1 : 2 mLs of Ethylacetate/IPA (85:15) (v:v)
Evaporate: :Evaporate to dryness
Reconstitute :1 mL 10% acetonitrile
Notes :Evaporate eluant to dryness under stream of N2 (50ºC). Reconstitute samples with 1mL of 10% acetonitrile

Application Detail (App ID: 19724)

Part No:00D-4462-AN
Dimensions:100 x 2.1 mm ID
Elution Type:Gradient
Elution A:5mM Ammonium acetate
Elution B:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
2 0 90 10
3 10 55 45
4 10.01 10 90
5 12 10 90
6 12.01 90 10
7 16 90 10

Flow Rate:400 µL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ amu (22°C)
Detector Info: AB Sciex API 5000™
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
Phenobarbital-d5
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2
Phenobarbital
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3
Butalbital-d5

4
Butalbital
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5
Pentobarbital
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6
Amobarbital
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7
Secobarbital
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