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Nicotine and its Metabolites in Urine By LC/MS/MS Using Strata-X-C and Gemini-NX C18

Sample Preparation Details

Part No:8B-S029-UBJ
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 60 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Dry:5min at full vacuum
Final Prep and Analysis: Inject: 10 µL on HPLC / Mass Spectrometer (MS) @ amu (550 °C)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Nornicotine 101.5
2 3-Hydroxycotinine 103.1
3 Anabasine 97.03
4 Cotinine 94.8
5 Nicotine 96.13

Application Detail (App ID: 19734)

Part No:00B-4453-B0
Dimensions:50 x 2 mm ID
Elution Type:Gradient
Elution A:20mM Ammonium bicarbonate
Elution B:100% Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 3 25 75
3 3.1 90 10
4 5 90 10

Flow Rate:500 µL/min
Col. Temp: 25 °C
Detection: Mass Spectrometer (MS) @ amu (550°C)
Detector Info: AB Sciex API 4000™
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Gemini-NX C18 4 x 2.0mm ID, 10/Pk Part No.: AJ0-8367
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Nornicotine
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2
3-Hydroxycotinine
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3
Anabasine
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4
Cotinine
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5
Nicotine
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