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Nicotine and its Metabolites in Urine By LC/MS/MS Using Strata-X-C and Gemini-NX C18

Sample Preparation Details

Condition 1 :2 mL 100% Methanol at 2 mL/min
Condition 2 :2 mL 20mM Ammonium acetate at 2 mL/min
Load :Load 0.50 mL Sample diluted in 0.5 mL 20mM Ammonium acetate
Wash 1 :2 mL 20mM Ammonium acetate at 2 mL/min
Wash 2 :2 mL 30% Methanol at 2 mL/min
Dry :5min at full vacuum
Elution 1 :4 mL 1.5% Ammonium hydroxide methonal solution 2x2 mL at 2 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :500 µL 10:90 Acentonitrile:20mM Ammonium bicarbonate

Application Detail (App ID: 19734)

Part No:00B-4453-B0
Dimensions:50 x 2 mm ID
Elution Type:Gradient
Elution A:20mM Ammonium bicarbonate
Elution B:100% Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 3 25 75
3 3.1 90 10
4 5 90 10

Flow Rate:500 µL/min
Col. Temp: 25 °C
Detection: Mass Spectrometer (MS) @ amu (550°C)
Detector Info: AB Sciex API 4000™
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Gemini-NX C18 4 x 2.0mm ID, 10/Pk Part No.: AJ0-8367
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Nornicotine
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2
3-Hydroxycotinine
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3
Anabasine
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4
Cotinine
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5
Nicotine
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