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Applications
Hi Temp Stability - Injection on new Aeris WIDEPORE column
19832
Separation Mode: Reversed Phase
Reversed Phase
WIDEPORE XB-C18
HPLC
Pharmaceutical/Biopharmaceutical
Life Science
Chromatographic Property
Biochemical Compound / Nutrient
Therapeutic / Clinical
Biochemical Compound / Nutrient
Biochemical Compound / Nutrient
Therapeutic / Clinical

Applications

Hi Temp Stability - Injection on new Aeris WIDEPORE column
Analytes
1Apomyoglobin

Analyte not found

Details
LC Conditions (App ID: 19832)
Column:
Brand Name:
Part No:
Phase Name:
WIDEPORE XB-C18
Elution Type:
Gradient
Mobile Phase:
A: Water with 0.1% TFA
B: Acetonitrile with 0.1% TFA
Gradient Profile:
Step No.Time(min)%A%B
10973
23973
3233565
Flow Rate:
0.312 mL/min
Temperature:
90°C
Detection:
UV-Vis Abs.-Diode Array (PDA) @ 214 nm (22°C)
Sample Note:
Application was actually run on a 50x4.6mm ID running at a flow rate of 1.5 mL/min. 50 x 4.6 mm which is not a commercially available part number. Information switched to 50x2.1mm for those that wish to replicate method.

Order Items Used in This Application

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