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Aldosterone on Kinetex 2.6 um XB-C18 50x3 mm ID

Sample Preparation Details

Part No:8B-S123-UBJ
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 60 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:Into individually labeled test tubes, combine 0.5 mL serum (or calibrator or other QC sample), 1 mL 25 mM Ammoniun bicarbonate (pH 8.9-9.0), and 0.1 mL working internal standard.
Dry:5 min at high vacuum
Final Prep and Analysis: Inject: 30 µL on HPLC / Tandem Mass Spec (MS-MS) @ (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Aldosterone
2 Cortisone

Application Detail (App ID: 20009)

Part No:00B-4496-Y0
Dimensions:50 x 3 mm ID
Elution Type:Gradient
Elution A:DI Water
Elution B:Methanol/Acetonitrile (50:50)
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 70 30
2 4 10 90
3 5 10 90
4 5.1 70 30
5 7 70 30

Flow Rate:500 µL/min
Col. Temp: ambient
Detection: Tandem Mass Spec (MS-MS) @ (22°C)
Detector Info: AB Sciex API 5000™
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 3.0mm ID Columns, 3/Pk Part No.: AJ0-8775
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
Aldosterone
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2
Cortisone
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