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Applications

Aldosterone on Kinetex 2.6 um XB-C18 50x3 mm ID

Sample Preparation Details

Condition 1 :3 mL Methanol
Condition 2 :3 mL 25 mM Ammonium bicarbonate
Load :Load 1.50 mL Sample diluted in 25 mM Ammonium bicarbonate (pH 8.9-9.0)
Wash 1 :3 mL 25 mM Ammonium bicarbonate
Wash 2 :3 mL 25% Methanol in water
Dry :5 min at high vacuum
Elution 1 :1 mL Ethyl acetate/Isopropanol/Ammonium hydroxide (7:2:1)
Elution 2 :1 mL Ethyl acetate/Isopropanol/Ammonium hydroxide (7:2:1)
Evaporate: :Evaporate to dryness
Reconstitute :100 µL Methanol/Water (30:70)

Application Detail (App ID: 20009)

Part No:00B-4496-Y0
Dimensions:50 x 3 mm ID
Elution Type:Gradient
Elution A:DI Water
Elution B:Methanol/Acetonitrile (50:50)
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 70 30
2 4 10 90
3 5 10 90
4 5.1 70 30
5 7 70 30

Flow Rate:500 µL/min
Col. Temp: ambient
Detection: Tandem Mass Spec (MS-MS) @ (22°C)
Detector Info: AB Sciex API 5000™
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 3.0mm ID Columns, 3/Pk Part No.: AJ0-8775
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
Aldosterone
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2
Cortisone
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