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Alfatoxins in Food by LC/MS/MS

Sample Preparation Details

Part No:8B-S013-HBJ
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 500 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:Pre-Treatment: To 5 g of peanut buffer, add 40 mL of Methanol/Water (80:20) containing 0.2 g of sodium chloride. Mechanically stir for 2 hours then filter out residual solids using Whatman filter paper.
Final Prep and Analysis: Inject: 10 µL on HPLC / Tandem Mass Spec (MS-MS) @ (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Aflatoxin M1
2 Aflatoxin G2
3 Aflatoxin G1
4 Aflatoxin B2
5 Aflatoxin B1

Application Detail (App ID: 20016)

Part No:00B-4447-B0
Dimensions:50 x 2 mm ID
Elution Type:Isocratic
Elution A:5mM Ammonium Acetate + 0.1% Formic Acid
Elution B:Methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 40 60

Flow Rate:0.4 mL/min
Col. Temp: 28 °C
Detection: Tandem Mass Spec (MS-MS) @ (0°C)
Detector Info: 3200 QTRAP
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, PFP 4 x 2.0mm ID, 10/Pk Part No.: AJ0-8326
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Aflatoxin M1
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2
Aflatoxin G2
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3
Aflatoxin G1
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4
Aflatoxin B2
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5
Aflatoxin B1
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