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Quantification of Carbamates Fungicides in Beverage by LC/MS/MS

Sample Preparation Details

Part No:8B-S100-TBJ
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:Combine 400 uL apple juice with 1 mL 25 mM Ammonium acetate buffer (pH 5.5), vortex
Dry:2 min at 10mm Hg
Final Prep and Analysis: Inject: 10 µL on HPLC / Tandem Mass Spec (MS-MS) @ (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Aldicarb-sulfone 40  140.8
2 Methomyl 40  107
3 3-Hydroxycarbofuran 40  146.2
4 Oxamyl 40  143.3
5 Aldicarb 40  79.7
6 Carbofuran 40  102
7 Carbofuran-d3 40 
8 Methiocarb 40  75.8

Application Detail (App ID: 20025)

Part No:00B-4439-B0
Dimensions:50 x 2 mm ID
Elution Type:Gradient
Elution A:5mM ammonium formate
Elution B:5mM ammonium formate in acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 85 15
2 2.5 5 95
3 4 5 95
4 4.01 85 15
5 8 85 15

Flow Rate:0.5 mL/min
Col. Temp: 28 °C
Detection: Tandem Mass Spec (MS-MS) @ (22°C)
Detector Info: 3200 QTRAP
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Gemini C18 4 x 2.0mm ID, 10/Pk Part No.: AJ0-7596
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Aldicarb-sulfone
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2
Methomyl
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3
3-Hydroxycarbofuran
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4
Oxamyl
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5
Aldicarb
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6
Carbofuran
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7
Carbofuran-d3
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8
Methiocarb
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