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Chloramphenicol in Seafood and dairy products by LC/MS/MS

Sample Preparation Details

Condition 1 :3 mL Methanol at 1 mL/min
Condition 2 :3 mL Water at 1 mL/min
Load :Load 10.00 mL Sample diluted in Pretreated sample
Wash 1 :3 mL Water at 1 mL/min
Dry :5 minutes @ 10 in Hg Vaccum
Elution 1 :3 mL Ethyl Acetate at 1 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :500 µL 0.5 mL Water/Acetonitrile (80:20)

Application Detail (App ID: 20026)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:Water
Elution B:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 -2 95 5
2 0 95 5
3 2 95 5
4 2.01 5 95
5 4.5 5 95

Flow Rate:0.4 mL/min
Col. Temp: 20 °C
Detection: Tandem Mass Spec (MS-MS) @ (450°C)
Detector Info: AB Sciex API 4000™
Note:SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime.
- SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782
- Holder Part No.: AJ0-9000

Analyte Details

Analyte Detail not available

1
Chloramphenicol
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2
Chloramphenicol
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3
Chloramphenicol-d5
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