For security purposes
FOR SECURITY PURPOSES - because Internet Explorer is no longer supported by Microsoft, we suggest that you interact with our secure site through one of our supported browsers - Google Chrome, Firefox, or MS Edge. If you continue to use this website with Internet Explorer you do so at your own risk and you may encounter problems.
Applications
Tetracyclines in Meat by LC/MS/MS
20032
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Antimalarials
Pharmaceutical
Therapeutic / Clinical

Applications

Tetracyclines in Meat by LC/MS/MS
Analytes
1Oxytetracycline
2Tetracycline
3Chlortetracycline
4Methacycline
5Doxycycline
Compound Name
Oxytetracycline
CID
5280972
Molecular Formula
C22H24N2O9
Molecular Weight
460
No. Hydrogen Bond Acceptors
11
No. Hydrogen Bond Donors
7
Details
LC Conditions (App ID: 20032)
Column:
Brand Name:
Part No:
Phase Name:
C18
Elution Type:
Gradient
Mobile Phase:
A: 0.1% Formic Acid in Water
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
10982
22982
32.18515
46.55050
57.10100
680100
78.1982
810982
Flow Rate:
0.8 mL/min
Temperature:
40°C
Detection:
Tandem Mass Spec (MS-MS) (600°C)
Detector Info:
3200 QTRAP LC/MS/MS
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Gemini C18 4 x 3.0mm ID, 10/Pk Part No.: AJ0-7597 - Holder Part No.: KJ0-4282
Sample Preparation Details
Description:
Strata-X 33 µm Polymeric Reversed Phase, 60 mg / 3 mL, Tubes, 50/Pk
Part Number:
Condition 1:
Methanol
Condition 2:
0.1% Formic Acid
Wash 1:
0.1% Formic Acid
Evaporation:
Evaporate to dryness
1
Condition
Methanol
2
Condition
0.1% Formic Acid
1
Shake
Methanol
1
Condition
Methanol

Order Items Used in This Application

Similar Applications
search
No Data