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Multiclass Antibiotics Screening of Kidney Juice, Serum, Milk and Honey by LC/MS/MS

Application Detail (App ID: 20033)

Part No:00B-4439-B0
Dimensions:50 x 2 mm ID
Elution Type:Gradient
Elution A:Water + 0.1% Formic Acid
Elution B:Methanol + 0.1% Formic Acid
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 98 2
2 7.27 20 80
3 7.37 1 99
4 11 98 2
5 15 98 2
6 11 98 2
7 15 98 2

Flow Rate:0.5 mL/min
Col. Temp: 40 °C
Detection: Tandem Mass Spec (MS-MS) @ (600°C)
Detector Info: AB Sciex 4000 QTRAP®
Note:Sample Prep Steps:

1. Weigh 1 g of homogenized beef kidney sample, kidney juice, or serum into a 50 mL FEP (fluorinated ethylene propylene) tube. Alternatively you can use a disposable polypropylene Corning tube.
2. Add 5 µL of the internal standard work solution.
3. Add 2 mL of water and 8 mL of acetonitrile
4. Mix briefly using a vortex mixer, and then shake for five minutes.
5. Centrifuge at 3450 rcf for five minutes.
6. Decant the supernatant into a 50 mL tube with 500 mg of Sepra C18-E sorbent.
7. Mix briefly using a vortex mixer and shake for 30 seconds.
8. Centrifuge at 3450 rcf for one minute.
9. Place a 5 mL aliquot of the supernatant into a graduated tube.
10. Evaporate down to less than 1 mL.
11. Make up the volume to 1 mL with water.
12. Filter the extract through a 0.45 µm Phenex™ RC syringe filter (part no. AF0-8103-12) and then transfer to a Verex™ autosampler vial (part no. AR0-9925-13).

Note: The extracts are now ready for LC/MS/MS analysis.


SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Gemini C18 4 x 2.0mm ID, 10/Pk Part No.: AJ0-7596
- Holder Part No.: KJ0-4282


Analyte Details

Analyte Detail not available

1
Florfenicol amine
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2
Sulfanilamide
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3
Sulfaguanidine
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4
Tetramisole
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5
Amoxicillin
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6
Sulfacetamide
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7
Lincomycin
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8
Metronidazole
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9
Dimetridazole
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10
Albendazole amino sulfone

11
Ronidazole
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12
Minocycline
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13
Sulfadiazine
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14
Thiabendazole
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15
Sulfathiazole
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16
Sulfapyridine
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17
Desethylene ciprofloxacin
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18
Ractopamine
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19
Ofloxacin
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20
Sulfamerazine
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21
Enoxacin
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22
Tetracycline
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23
Norfloxacin
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24
Ampicillin
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25
Ciprofloxacin
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26
Thiamphenicol
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27
Lomefloxacin
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28
Enrofloxacin
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29
Oxytetracycline
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30
Danofloxacin
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31
Orbifloxacin
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32
Difloxacin
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33
Sarafloxacin
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34
Sulfamethazine-d4
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35
Sulfamethazine
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36
2-mercaptobenzimidazole
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37
Sulfamethizole
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38
Ipronidazole-OH
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Sulfamethoxypyridazine
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40
Spiramycin
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41
Chlorotetracycline
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42
Sulfachloropyridazine
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43
Sulfamethoxazole
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44
Florfenicol
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45
Sulfadoxine
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46
Clindamycin
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47
Tilmicosin
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48
Sulfisoxazole
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49
Carbadox
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50
6-phenyl-2-thiouracil
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51
Albendazole sulfoxide
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52
Chloramphenicol
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53
Doxycycline
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54
Bacitracin
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2-Quinoxalinecarboxylic acid
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56
Oxolinic acid
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57
Sulfadimethoxine
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58
Albendazole sulfone
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59
Erythomycin
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60
Sulfaquinoloxaline
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61
Tylosin
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Fenbendazole sulfoxide
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Josamycin
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64
Fenbendazole sulfone

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Albendazole
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66
Flumequine
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67
Sulfanitran
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68
Ceftiofur
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69
Mebendazole
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70
Oxyphenbutazone
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71
Fenbendazole
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72
Ketoprofen
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73
Penicillin G
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74
Naproxen
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75
Phenylbutazone
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76
Flunixin
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77
Nicarbazin
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78
Etodolac
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79
Dipyrone
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80
Cloxacillin
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81
Ibuprofen
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82
Diclofenac
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83
Indomethacin
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84
Triclabendazole
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85
Tolfenamic acid
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86
Novobiocin
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87
Niclosamide
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88
Decoquinate
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89
Sulfasalazine
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90
Monensin
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91
Narasin
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92
Lasalocid
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93
Lasalocid
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94
Rafoxanide
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