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Sulfonamides in Meat by LC/MS/MS

Sample Preparation Details

Part No:8B-S100-UBJ
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 60 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Dry:2min at 10inches Hg
Final Prep and Analysis: Inject: 50 µL on HPLC / Tandem Mass Spec (MS-MS) @ (300 °C)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Sulfadiazine
2 Sulfathiazole
3 Sulfapyridine
4 Sulfamerazine
5 Sulfamethazine
6 Sulfamethoxazole
7 Sulfadimethoxine
8 Sulfaquinoxaline

Application Detail (App ID: 20036)

Part No:00B-4439-E0
Dimensions:50 x 4.6 mm ID
Elution Type:Gradient
Elution A:0.1% Formic Acid in Water
Elution B:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 98 2
2 2 98 2
3 2.1 85 15
4 6.5 50 50
5 7.1 0 100
6 8 0 100
7 8.1 98 2
8 10 98 2

Flow Rate:800 µL/min
Col. Temp: ambient
Detection: Tandem Mass Spec (MS-MS) @ (300°C)
Detector Info: AB Sciex 3200 QTRAP®
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Gemini C18 4 x 3.0mm ID, 10/Pk Part No.: AJ0-7597
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Sulfadiazine
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2
Sulfathiazole
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3
Sulfapyridine
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4
Sulfamerazine
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5
Sulfamethazine
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6
Sulfamethoxazole
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7
Sulfadimethoxine
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8
Sulfaquinoxaline
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