Underivatized MMA and Succinic Acid in Human Urine on Gemini C18, 3u, 100x3 mm

Sample Preparation Details

Part No:8B-S038-TAK
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Load:Into individually labeled 1.5 mL conical micro-centrifuge tubes combine 0.5 mL 25 mM Ammonium formate, 50 uL IS and 100 uL blank, standard, or sample
Dry:Dry under high vacuum for 5-10 min
Final Prep and Analysis: This method is for the analysis of underivatized MMA. Inject: 10 µL on HPLC / Tandem Mass Spec (MS-MS) @ (ambient)


Name Spiked
Log P pKa % Rec %RSC (n=0)
1 Succinic acid
2 Methylmalonic acid

Application Detail (App ID: 20586)

Part No:00D-4439-Y0
Dimensions:100 x 3 mm ID
Elution Type:Gradient
Elution A:0.1% Formic Acid in DI H2O
Elution B:0.1% Formic Acid + 10 mM Ammonium Formate in MeOH
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 85 15
2 1.5 5 95
3 2.5 5 95
4 2.51 85 15
5 4.5 85 15

Flow Rate:700 µL/min
Col. Temp: 40 °C
Detection: Tandem Mass Spec (MS-MS) @ (22°C)
Detector Info: AB Sciex API 4000™ (Negative Mode ESI)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Gemini C18 4 x 2.0mm ID, 10/Pk Part No.: AJ0-7596
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

Succinic acid

Methylmalonic acid