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Applications

Underivatized MMA and Succinic Acid in Human Urine on Gemini C18, 3u, 100x3 mm

Sample Preparation Details

Condition 1 :1 mL Methanol
Condition 2 :1 mL 25 mM Ammonium Formate
Load :Load Sample
Wash 1 :0.5 mL Methanol/Water (50:50)
Dry :Dry under high vacuum for 5-10 min
Elution 1 :0.6 mL Ammonium hydroxide/Methanol (2:98)
Elution 2 :0.6 mL Ammonium hydroxide/Methanol (2:98)
Evaporate: :Evaporate to dryness
Reconstitute :200 µL 0.1% Formic acid
Notes :This method is for the analysis of underivatized MMA.

Application Detail (App ID: 20586)

Part No:00D-4439-Y0
Dimensions:100 x 3 mm ID
Elution Type:Gradient
Elution A:0.1% Formic Acid in DI H2O
Elution B:0.1% Formic Acid + 10 mM Ammonium Formate in MeOH
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 85 15
2 1.5 5 95
3 2.5 5 95
4 2.51 85 15
5 4.5 85 15

Flow Rate:700 µL/min
Col. Temp: 40 °C
Detection: Tandem Mass Spec (MS-MS) @ (22°C)
Detector Info: AB Sciex API 4000™ (Negative Mode ESI)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Gemini C18 4 x 2.0mm ID, 10/Pk Part No.: AJ0-7596
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Succinic acid
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2
Methylmalonic acid
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