| 1Aldosterone |
|---|
Compound Name Aldosterone |
CID 5839 |
Molecular Formula C21H28O5 |
Molecular Weight 360 |
No. Hydrogen Bond Acceptors 5 |
No. Hydrogen Bond Donors 2 |
Applications
Aldosterone analysis by LCMS using Kinetex 2.6um XB-C18 50x3.0mm
20648
Separation Mode: Reversed Phase
Reversed Phase
XB-C18
HPLC
Clinical
Clinical Research
Therapeutic / Clinical
Analytes
Details
LC Conditions (App ID: 20648)
Column: | ||||||||||||||||||||||||
Brand Name: Kinetex | ||||||||||||||||||||||||
Part No: | ||||||||||||||||||||||||
Phase Name: XB-C18 | ||||||||||||||||||||||||
Elution Type: Gradient | ||||||||||||||||||||||||
Mobile Phase: A: Water B: 50/50 AcCN/Methanol | ||||||||||||||||||||||||
Gradient Profile:
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Flow Rate: 0.5 mL/min | ||||||||||||||||||||||||
Temperature: 22°C | ||||||||||||||||||||||||
Detection: Tandem Mass Spec (MS-MS) (22°C) |
Sample Preparation Details
Description: Strata-X-A 33 µm Polymeric Strong Anion, 60 mg / 3 mL, Tubes, 50/Pk |
Part Number: |
Condition 1: Methanol |
Condition 2: 25 mM Ammonium Bicarbonate |
Wash 1: 25mm ammonium bicarbonate buffer |
Wash 2: 25% methanol in water |
Evaporation: Evaporate to dryness |
1 Condition Methanol |
2 Shake Ethyl Acetate:Isopropanol:NH4OH (7:2:1) |
1 Condition Methanol |
2 Shake Ethyl Acetate:Isopropanol:NH4OH (7:2:1) |
1 Condition Methanol |
2 Shake Ethyl Acetate:Isopropanol:NH4OH (7:2:1) |
Order Items Used in This Application
00B-4496-Y0
Kinetex 2.6 µm XB-C18 100 Å, LC Column 50 x 3 mm, Ea
Qty:
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Aldosterone
Kinetex 2.6 µm XB-C18 100 Å, LC Column 50 x 3 mm, Ea
Reversed Phase
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