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Aldosterone analysis by LCMS using Kinetex 2.6um XB-C18 50x3.0mm

Sample Preparation Details

Part No:8B-S123-UBJ
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 60 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Dry:5 min at high vacuum
Final Prep and Analysis: Inject: 30 µL on HPLC / Tandem Mass Spec (MS-MS) @ (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 Aldosterone

Application Detail (App ID: 20648)

Part No:00B-4496-Y0
Dimensions:50 x 3 mm ID
Elution Type:Gradient
Elution A:Water
Elution B:50/50 AcCN/Methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 70 30
2 4 10 90
3 5 10 90
4 5.1 70 30
5 7 70 30

Flow Rate:0.5 mL/min
Col. Temp: ambient
Detection: Tandem Mass Spec (MS-MS) @ (22°C)

Analyte Details

Analyte Detail not available

1
Aldosterone
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