Region

Applications

Histamine by LCMS using Luna 3um HILIC 100x2.1mm

Sample Preparation Details

Part No:8B-S035-TBJ
Solid Phase Extraction (SPE) procedure:Note: The solvent volumes shown below are for a 30 mg bed mass. The solvent volumes will need to be adjusted for a smaller or larger bed mass.
Dry:2 min at high vacuum level, ~20-30 in Hg
Final Prep and Analysis: Inject: 2 µL on HPLC / Tandem Mass Spec (MS-MS) @ (ambient)

Analytes:

Name Spiked
Conc.
Log P pKa % Rec %RSC (n=0)
1 1-Methyl histamine
2 N-Methyl histamine
3 Histamine

Application Detail (App ID: 20650)

Part No:00D-4449-B0
Dimensions:100 x 2 mm ID
Elution Type:Gradient
Elution A:50:45:5 ACN:DI H2O: 100 mM Amm. Form, pH 3.22
Elution B:95:5 ACN:100 mM Amm. Formate, pH 3.22
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 17 83
2 1.5 100 0
3 2.5 100 0
4 2.6 17 83
5 4.5 17 83

Flow Rate:0.6 mL/min
Col. Temp: ambient
Detection: Tandem Mass Spec (MS-MS) @ (22°C)
Detector Info: AB Sciex API 5000™

Analyte Details

Analyte Detail not available

1
1-Methyl histamine

2
N-Methyl histamine

3
Histamine
search