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Histamine by LCMS using Luna 3um HILIC 100x2.1mm

Sample Preparation Details

Condition 1 :1 mL Methanol
Condition 2 :1 mL DI water
Load :Load Sample
Wash 1 :2 mL DI water
Wash 2 :2 mL MeOH
Dry :2 min at high vacuum level, ~20-30 in Hg
Elution 1 :0.5 mL 87:13 B:A, fortified to contain 5% formic acid
Evaporate: :Evaporate to dryness
Reconstitute :0 µL initial mobile phase

Application Detail (App ID: 20650)

Part No:00D-4449-B0
Dimensions:100 x 2 mm ID
Elution Type:Gradient
Elution A:50:45:5 ACN:DI H2O: 100 mM Amm. Form, pH 3.22
Elution B:95:5 ACN:100 mM Amm. Formate, pH 3.22
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 17 83
2 1.5 100 0
3 2.5 100 0
4 2.6 17 83
5 4.5 17 83

Flow Rate:0.6 mL/min
Col. Temp: ambient
Detection: Tandem Mass Spec (MS-MS) @ (22°C)
Detector Info: AB Sciex API 5000™

Analyte Details

Analyte Detail not available

1
1-Methyl histamine
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2
N-Methyl histamine
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3
Histamine
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