| 11-Methyl histamine |
|---|
| 2N-Methyl histamine |
| 3Histamine |
Compound Name 1-Methylhistamine |
CID 3614 |
Molecular Formula C6H11N3 |
Molecular Weight 125 |
No. Hydrogen Bond Acceptors 2 |
No. Hydrogen Bond Donors 1 |
Applications
Histamine by LCMS using Luna 3um HILIC 100x2.1mm
20650
Histamine Agents
Clinical
Biochemical Compound / Nutrient
Therapeutic / Clinical
Analytes
Details
LC Conditions (App ID: 20650)
Elution Type: Gradient | ||||||||||||||||||||||||
Mobile Phase: A: 50:45:5 ACN:DI H2O: 100 mM Amm. Form, pH 3.22 B: 95:5 ACN:100 mM Amm. Formate, pH 3.22 | ||||||||||||||||||||||||
Gradient Profile:
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Flow Rate: 0.6 mL/min | ||||||||||||||||||||||||
Temperature: 22°C | ||||||||||||||||||||||||
Detection: Tandem Mass Spec (MS-MS) (22°C) | ||||||||||||||||||||||||
Detector Info: SCIEX API 5000™ |
Sample Preparation Details
Description: Strata-X-CW 33 µm Polymeric Weak Cation, 30 mg / 3 mL, Tubes, 50/Pk |
Part Number: |
Condition 1: Methanol |
Condition 2: DI water |
Wash 1: DI water |
Wash 2: MeOH |
Evaporation: Evaporate to dryness |
1 Condition Methanol |
2 Condition DI water |
1 Shake 87:13 B:A, fortified to contain 5% formic acid |
1 Wash DI water |
2 Condition DI water |
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1-Methyl histamine
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